Implementation of stimulated Raman scattering microscopy for single cell analysis

Publication date: 16 Mag 2017

ConferenceSource: LEGACY

In this work, we present successfully realization of a nonlinear microscope, not purchasable in commerce, based on stimulated Raman scattering. It is obtained by the integration of a femtosecond SRS spectroscopic setup with an inverted research microscope equipped with a scanning unit. Taking account of strength of vibrational contrast of SRS, it provides label-free imaging of single cell analysis. Validation tests on images of polystyrene beads are reported to demonstrate the feasibility of the approach. In order to test the microscope on biological structures, we report and discuss the label-free images of lipid droplets inside fixed adipocyte cells.

Publisher
International Society for Optics and Photonics
Origin
Nonlinear Optics and Applications X
Legacy ID
3c05c4e2b0fe56b06f7d3bffe3d2b80f
Biblio references
Volume: 10228 Pages: 102280S