The fluorescence enhancement mechanism was investigated in the presence of distance-dependent dipole metal nanoantennas using Alexa Fluor-647 as a probe molecule commonly used in biological contexts. Guided by numerical modeling, a planar arrangement of non-interacting Au nanodiscs arrays was realized on glass substrates by a cost-effective nanofabrication technique, and the contribution of their optical properties to the emission properties of the dye monolayer is evaluated and compared to the bare glass substrates. Remarkably, at an optimal spacer thickness of nearly 10 nm, a maximum fluorescence enhancement factor of up to 180 was observed. The knowledge gained will greatly assist in the design of efficient photonic devices utilizing this technology.